Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: circRNAs expression profiles detected by microarray in the AAA group and control group. a The box plot shows the nearly identical distributions of normalized intensity values from the aortic samples of the AAA and control group. b The scatter plot is built to assess the expression variation of circRNAs between the two groups. The X and Y axes indicate the normalized intensity values of each circRNAs from the AAA and control group. The dots above the upper green line and below the lower green line represent the dysregulated circRNAs with a fold change (FC) > 2.0 between the two groups. c The volcano plot presents differentially expressed circRNAs in AAA. The vertical lines correspond to 2-fold upregulation and downregulation, the horizontal line indicates P value of 0.05. The red dots represent the differentially expressed circRNAs (FC > 2.0 and P value < 0.05). d Hierarchical clustering analysis reveals a distinguishable expression profile of circRNAs between the AAA and control group. Each column indicates an aortic sample, each row represents a circRNA. The red and green color indicate high and low expression level, respectively. e Chromosomal distribution of the differentially expressed circRNAs between the two groups

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Expressing, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: Validation of six randomly selected dysregulated circRNAs by qRT-PCR. Each circRNA was evaluated at least three times and compared with the results of microarray. The Y axis indicates the fold change of AAA vs control of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Biomarker Discovery, Quantitative RT-PCR, Microarray, Control

Journal: BMC Cardiovascular Disorders

Article Title: Circular RNA expression profile and its potential regulative role in human abdominal aortic aneurysm

doi: 10.1186/s12872-020-01374-8

Figure Lengend Snippet: The predicted circRNA/miRNA interaction networks for six randomly selected circRNAs. a , b The red nodes indicate upregulated circRNAs. c - f The blue nodes represent downregulated circRNAs. The green nodes are five complementary binding miRNAs of each circRNA

Article Snippet: The fragmented labeled cRNAs were hybridized onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2).

Techniques: Binding Assay

Journal: Archives of Medical Science : AMS

Article Title: Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

doi: 10.5114/aoms.2020.91290

Figure Lengend Snippet: Microarray analysis was applied to detect the lncRNAs and mRNAs in glioma compared to normal peritumoral tissue. A – Differentially expressed lncRNAs were detected in gliomas. A, B – Differentially expressed mRNAs were detected in gliomas. C – Clustering data of lncRNAs in gliomas were analyzed. D – Clustering data of mRNAs in gliomas were analyzed

Article Snippet: The synthesized cDNAs were labeled and hybridized to Arraystar Human lncRNA Microarray V2.0 (Arraystar, Rockville, MD) containing probes for 33,045 lncRNAs and 30,215 mRNAs identified from both publications and authoritative databases, such as RefSeq, UCSC Knowngenes, and Ensembl.

Techniques: Microarray

Journal: Archives of Medical Science : AMS

Article Title: Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

doi: 10.5114/aoms.2020.91290

Figure Lengend Snippet: Summary of data from microarray for three pairs of glioma and adjacent normal tissues

Article Snippet: The synthesized cDNAs were labeled and hybridized to Arraystar Human lncRNA Microarray V2.0 (Arraystar, Rockville, MD) containing probes for 33,045 lncRNAs and 30,215 mRNAs identified from both publications and authoritative databases, such as RefSeq, UCSC Knowngenes, and Ensembl.

Techniques: Microarray, RNA Expression

Journal: Archives of Medical Science : AMS

Article Title: Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

doi: 10.5114/aoms.2020.91290

Figure Lengend Snippet: LncRNA-mRNA co-expression network: nodes with red cycle represent lncRNAs, nodes without cycle represent mRNAs, straight lines represent interactions between genes, purple represents increased expression, and blue represents decreased expression. The size of the node represents the degree; the higher the degree, the more genes interact with the particular node in the network

Article Snippet: The synthesized cDNAs were labeled and hybridized to Arraystar Human lncRNA Microarray V2.0 (Arraystar, Rockville, MD) containing probes for 33,045 lncRNAs and 30,215 mRNAs identified from both publications and authoritative databases, such as RefSeq, UCSC Knowngenes, and Ensembl.

Techniques: Expressing

Journal: Archives of Medical Science : AMS

Article Title: Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

doi: 10.5114/aoms.2020.91290

Figure Lengend Snippet: Degree was used to assess interactions in the lncRNA/mRNA network. This table is a collection of a series of key lncRNA/mRNAs

Article Snippet: The synthesized cDNAs were labeled and hybridized to Arraystar Human lncRNA Microarray V2.0 (Arraystar, Rockville, MD) containing probes for 33,045 lncRNAs and 30,215 mRNAs identified from both publications and authoritative databases, such as RefSeq, UCSC Knowngenes, and Ensembl.

Techniques:

Journal: Archives of Medical Science : AMS

Article Title: Aberrant expression of long non-coding RNAs (lncRNAs) is involved in brain glioma development

doi: 10.5114/aoms.2020.91290

Figure Lengend Snippet: Comparison of microarray data and qPCR results. A – qPCR was used to verify expression of lncRNAs ak125809, ak098473, uc002ehu.1, bc043564, NR_027322, and uc003qmb.2. B – Distribution of lncRNA expression levels were provided. All six lncRNAs of ak125809, ak098473, uc002ehu.1, bc043564, NR_027322, and uc- 003qmb.2 were validated by qPCR analysis in the 40 paired glioma and peritumoral tissues. Each histogram represents the average fold change (T/N) with logarithmic conversion. Error bars are indicative of standard deviation. Distribution of lncRNA expression

Article Snippet: The synthesized cDNAs were labeled and hybridized to Arraystar Human lncRNA Microarray V2.0 (Arraystar, Rockville, MD) containing probes for 33,045 lncRNAs and 30,215 mRNAs identified from both publications and authoritative databases, such as RefSeq, UCSC Knowngenes, and Ensembl.

Techniques: Comparison, Microarray, Expressing, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Schematic depicting the composition of circRNA_101237 (encoded by CDK8). circRNA, circular RNA; CDK8, cyclin-dependent kinase 8; chr, chromosome.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: CircRNA_101237 expression in HCC tissues. (A) Heatmap for differentially expressed circRNAs in HCC tumor tissues and the matched adjacent tissues (n=3). (B) RT-qPCR was performed to determine the expression of circRNA_101237 in tumor tissues (n=100) and adjacent tissues (n=100). (C) RT-qPCR was performed to assess the expression of circRNA_101237 in serum samples from patients with HCC (n=234) and healthy controls (n=120). (D) Kaplan-Meier survival curves were drawn to compare the survival of patients with high circRNA_101237 expression (n=148) and those with low circRNA_101237 expression (n=86). RT-qPCR, reverse transcription-quantitative PCR; HCC, hepatocellular carcinoma; circRNA_101237, circular RNA 101237; hsa, Homo sapiens.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Clinical association between serum circRNA_101237 levels and clinicopathological characteristics of patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Univariate analysis of prognostic factors for patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Multivariate analysis of independent prognostic factors for patients with hepatocellular carcinoma.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques:

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Expression of circRNA_101237 is associated with cisplatin resistance. (A) RT-qPCR was performed to assess the expression of circRNA_101237 in serum samples of patients with cisplatin-sensitive HCC (n=50) and those with cisplatin-resistant HCC (n=35). (B) RT-qPCR was performed to determine the expression of circRNA_101237 in liver cancer cells. * P<0.05 vs. HCCLM3 cells; #P<0.05, Huh7/DDP vs. Huh7. Huh/DDP, cisplatin-resistant Huh7 cells; RT-qPCR, reverse transcription-quantitative PCR; HCC, hepatocellular carcinoma; circRNA_101237, circular RNA 101237.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Cisplatin resistance-associated circRNA_101237 serves as a prognostic biomarker in hepatocellular carcinoma

doi: 10.3892/etm.2020.8526

Figure Lengend Snippet: Changes in circRNA_101237 expression in hepatocellular carcinoma cell lines in response to cisplatin treatment. (A) Cells were treated for 48 h with cisplatin at 0, 0.5, 1 and 2 µg/ml. (B) Cells were treated with cisplatin at 1 µg/ml for 0, 12, 24 or 48 h. Reverse transcription-quantitative PCR was performed to assess the expression of circRNA_101237. *P<0.05 vs. controls. circRNA_101237, circular RNA 101237.

Article Snippet: The circRNA microarray data were analyzed using Arraystar Human circRNA Array V2 analysis (Arraystar) by Kangchen BioTech Inc.

Techniques: Expressing, Real-time Polymerase Chain Reaction